Abstract
BackgroundTorque teno sus virus (TTSuV), infecting domestic swine and wild boar, is a non-enveloped virus with a circular, single-stranded DNA genome. which has been classified into the genera Iotatorquevirus (TTSuV1) and Kappatorquevirus (TTSuV2) of the family Anelloviridae. A molecular study was conducted to detect evidence of a phylogenic relationship between these two porcine TTSuV genogroups from the sera of 244 infected pigs located in 21 subordinate prefectures and/or cities of Sichuan.ResultsBoth genogroups of TTSuV were detected in pig sera collected from all 21 regions examined. Of the 244 samples, virus from either genogroup was detected in 203 (83.2%), while 44 animals (18.0%) were co-infected with viruses of both genogroups. Moreover, TTSuV2 (186/244, 76.2%) was more prevalent than TTSuV1 (61/244, 25%). There was statistically significant difference between the prevalence of genogroups 1 infection alone (9.4%, 23/244) and 2 alone (64.8%, 158/244), and between the prevalence of genogroups 2 (76.2%, 186/244) and both genogroups co-infection (18.0%, 44/244). The untranslated region of the swine TTSuV genome was found to be an adequate molecular marker of the virus for detection and surveillance. Phylogenetic analysis indicated that both genogroups 1 and 2 could be further divided into two subtypes, subtype a and b. TTSuV1 subtype b and the two TTSuV2 subtypes are more prevalent in Sichuan Province.ConclusionsOur study presents detailed geographical evidence of TTSuV infection in China.
Highlights
Torque teno sus virus (TTSuV), infecting domestic swine and wild boar, is a non-enveloped virus with a circular, single-stranded DNA genome. which has been classified into the genera Iotatorquevirus (TTSuV1) and Kappatorquevirus (TTSuV2) of the family Anelloviridae
TTSuV prevalence among regional breeds of pigs Using nested-polymerase chain reaction (PCR) technique to amplify target fragments, we found that 83.2% (203/244) of domestic swine tested were positive for one of the TTSuV genogroups
A total of three tissue samples (3/20, 15%) from post-weaning multisystemic wasting syndromeaffected pigs and 30 blood samples (30/40, 75%) from healthy pigs were positive for Torque teno sus virus 1 (TTSuV1) and/or 2 (TTSuV2) [26]
Summary
Torque teno sus virus (TTSuV), infecting domestic swine and wild boar, is a non-enveloped virus with a circular, single-stranded DNA genome. which has been classified into the genera Iotatorquevirus (TTSuV1) and Kappatorquevirus (TTSuV2) of the family Anelloviridae. Torque teno sus virus (TTSuV), infecting domestic swine and wild boar, is a non-enveloped virus with a circular, single-stranded DNA genome. Which has been classified into the genera Iotatorquevirus (TTSuV1) and Kappatorquevirus (TTSuV2) of the family Anelloviridae. Torque teno sus virus (TTSuV) is a member of the Anelloviridae family. TTSuV is a small icosahedral, non-enveloped virus with a singlestranded (ss), negative sense, circular DNA genome which can infect domestic swine and wild boar [2]. Members of the Anelloviridae family can infect many vertebrate animals including humans, domestic swine, single pig [8]. These strains showed distinct genotypes or subtypes, a revised classification system for TTSuV was proposed. Nested polymerase chain reaction (PCR), based on the conserved regions in the UTR of TTSuV1 and TTSuV2, has been widely employed to detect these viruses [10,11,12]
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