Molecular investigation of Mecgene Among coagulase negative Staphylococcus isolated from different cases

Abstract

This study is aimed on investigating the coagulase negative Staphylococci (CONs) in human infections and mecA gene, that is responsible for some virulence factors. One hindered and fifty specimens were collected from different infection case theories, from the period February to June 2018, at AL – Diwanyiah Teaching Hospital. The specimens included urine, ear swab, vaginal swab, pus, wound swab, skin carrier, nasal, nipple, stool and tracheal. The specimens were cultured on blood agar and mannitol salt agar. identification was depended on gram stained and biochemical tests. Then final identification with APi staph system. Thirty three isolates identified coagulase negative staphylococci (CONs) which included S.lentus and S.xylosus. Twenty isolates were highly resistant to methicillin, oxacillin and cefoxitin. The PCR were used to detected the mecA gene. The results showed that 20 isolates had mecA gene. Some virulence factors of CONs were detected including hemolysin- determined in 8 isolates, urease producing - determined in 13 isolates.