Abstract
This study aimed to isolate and diagnose Salmonella typhi by flic gene and investigated the presence ofblaTEM1 gene by using PCR technique and electrophoresis systems in Najaf Province includes 40 clinicalspecimens blood for both sexes with an age ranged between )5-60) years old .The study result by using a flicgene was detected in 30 of the 40 isolates of S. typhi isolated from typhoid patients , which was conducted inthe diagnosis of bacteria concluded that the technique PCR are the most sensitive in the detection of S. typhicompare with other way, blaTEM-1 was detected in 16 of the 40 isolates from S.typhi . The conseder the bla TEMgene encoding TEM-1 b-lactamase is think to give resistance only to penicillins and early cephalosporins;thus , the spectrum resistance of TEM-1 descendants may extend to second, third and fourth-generationcephalosporins.
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