Abstract
Methicillin-resistant staphylococci may also be resistant to some other antibiotics as well as β-lactams. Co-existence of resistance to methicillin and macrolides was genetically investigated in Staphylococcus aureus. A total of 59 S. aureus strains isolated from nasal cavity of patients hospitalized in the pediatric service were investigated for their susceptibility to oxacillin and erythromycin. In addition, the β-lactams resistance gene (mecA and blaZ), the erythromycin resistance methylase genes (ermA, ermB, and ermC) and macrolide efflux genes msrA were analysed by multiplex PCR. The minimal inhibitory concentration (MIC) values revealed that 98 and 97% of isolates were resistant to oxacillin and erythromycin, respectively. In addition, the frequency of resistant strains for tested genes was 66% (mecA), 100% (blaZ), 15% (ermA), 30% (ermB), 22% (ermC) and 36% (msrA). A high rate of macrolide resistance was determined in methicillin-resistant staphylococci. The erm gene was the most frequently detected. Results of susceptibility testing and the carriage of resistance genes did not match. Thus, polymerase chain reaction (PCR) multiplex identification of antibiotic resistance genes, as a rapid and accurate technique, could be a supplementary method, which can be easily incorporated into the diagnostic molecular microbiology laboratory work flow. Key words: Staphylococcus aureus, multidrug resistance, multiplex polymerase chain reaction (PCR).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.