Molecular investigation and description of Iberozospeum n. gen., including the description of one new species (Eupulmonata, Ellobioidea, Carychiidae)

Abstract

The subterranean realm of the Cantabrian-Pyrenean region of northern Spain harbours a rich diversity of Zospeum. Due to their tiny size and the difficulty of finding them alive, scarce animal material has been available for scientific investigation. Recent investigations of Zospeum shells have provided valuable, but limited insights towards our understanding of the evolutionary processes occurring within this taxon in northern Spain. In an integrative study, we investigate 57 populations of Zospeum from northern Spanish caves using two mitochondrial (COI and 16S) and two nuclear markers (H3 and 5.8 S rRNA + ITS2). Revealed is a separate radiation of the northern Spanish species for which the new genus, Iberozospeum, is proposed. The independent radiation of Dinaric Zospeum from that of northern Spain justifies the designation of Iberozospeum n. gen. Morphological evidence is provided via histological analysis of Iberozospeum vasconicum and SEM analyses of radulae of eastern Alpine, Dinaric and Iberian species. Important differences in morphological structure and character states are presented, including the first view of the sexually mature female and the presence of the giant albumen gland in an individual of the subterranean, troglobitic Carychiidae. Significant differences are revealed in superficial crystallographic structure of the columellar lamellae, the morphology of the columellar muscle and in the radula. Radular ribbon length, ribbon broadness, straightness of the ribbon base and cusp configuration are distinctive in the Iberian species. One new species is described corroborated by genetic and morphological characters.