Abstract
BackgroundRevealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet.Materials and methodsqRT-PCR and western blot were conducted to measure relevant RNA and protein expressions. CCK-8, colony formation, EdU, flow cytometry, wound-healing, transwell assays and xenograft experiments were performed to determine the role of linc01134 in HCC. ChIP and luciferase reporter assays were performed to analyze the effects of Yin Yang-1 (YY1) on linc01134 transcription activity. Relevant mechanical experiments were performed to verify interaction between relative genes.ResultsYY1 enhanced linc01134 transcription by interacting with linc01134 promoter. Knockdown of linc01134 inhibited proliferation, migration and epithelial-mesenchymal transition (EMT), yet promoting apoptosis in HCC cells. Mechanically, linc01134 acted as miR-324-5p sponge and interacted with insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) to increase the stability of YY1 mRNA expression. Up-regulated YY1 continuously stimulated linc01134 expression by enhancing linc01134 promoter activity, forming a positive feedback loop.ConclusionLinc01134/miR-324-5p/IGF2BP1/YY1 feedback loop mediates HCC progression, which possibly provide prognosis and treatment target of HCC.
Highlights
Revealing the mechanical role of long non-coding RNAs in tumorigenesis can contribute to novel therapeutic target for cancers
We analyzed the expression of linc01134 in 46 paired collected hepatocellular carcinoma (HCC) tissues and normal tissue. Quantitative real-time polymerase chain reaction (qRT-PCR) result manifested that linc01134 was overtly up-regulated in HCC tissues (Fig. 1c)
We knocked down linc01134 in SNU-449 and HepG2 cells to study its influence on cellular behavior
Summary
Revealing the mechanical role of long non-coding RNAs (lncRNAs) in tumorigenesis can contribute to novel therapeutic target for cancers. The regulatory role of linc01134 in hepatocellular carcinoma (HCC) has not been studied yet. Advance in therapeutic methods has been made in recent years, the mortality of HCC still ranks the third among all cancers. Worse still, this rate is still on the rise worldwide [2]. The numerous non-protein-coding transcripts have been discovered to play important regulatory role in diseases, including cancers. Long non-coding RNAs (lncRNAs), which are over 200 nucleotides in length, have emerged as key regulators in biological processes
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