Abstract
Molecular interferometric imaging (MI2) is a common-path interferometric imaging technique for detecting protein binding to surfaces. The experimental metrology limit is 10 pm/pixel longitudinal resolution at 0.4-mum diffraction-limited lateral resolution, corresponding to 1.7 attogram of protein, which is only 8 antibody molecules per pixel, near to single-molecule detection. The scaling mass sensitivity at the metrology limit is 5 fg/mm. We demonstrate a protein microarray application in a 128-multiplex immunoassay. Assay applications include prostate specific antigen (PSA) at a detection limit of 60 pg/mL and the cytokine interleukin-5 (IL-5) at a detection limit of 50 pg/mL. Realtime binding assays using MI2 enable the study of reaction kinetics of antibodies exposed to antigen, and the binding of antibody Fc regions to protein G.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IEEE Journal of Selected Topics in Quantum Electronics
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
