Abstract
Potato yellow vein virus (PYVV) was detected in potatoes grown in the Central highlands, north of Bogotá (~3000 m altitude), Colombia. At this altitude viral whitefly vectors are largely absent, but infection persists because of the use of uncertified tubers. Plants with typical PYVV-induced yellowing symptoms, as well as with atypical yellowing or non-symptomatic symptoms were sampled at three separate geographical locations. PYVV presence was assessed by RT-PCR, and several plants were subjected to high-throughput sequencing (HTS) of their small RNA (sRNA) populations. Complete or almost complete sequences of four PYVV isolates were thus reconstructed, all from symptomatic plants. Three viral isolates infected plants singly, while the fourth co-infected the plant together with a potyvirus. Relative proportions of sRNAs to each of the three crinivirus genomic RNAs were found to remain comparable among the four infections. Genomic regions were identified as hotspots of sRNA formation, or as regions that poorly induced sRNAs. Furthermore, PYVV titres in the mixed versus single infections remained comparable, indicating an absence of synergistic/antagonistic effects of the potyvirus on the accumulation of PYVV. Daughter plants raised in the greenhouse from tubers of the infected, field-sampled plants displayed mild PYVV infection symptoms that disappeared with time, demonstrating the occurrence of recovery and asymptomatic infection phenotypes in this pathosystem.
Highlights
Potato yellow vein virus (PYVV) causes a disease known as potato yellow vein disease (PYVD) in potato crops in Colombia, Ecuador, Perú and Venezuela [1], with crop losses estimated at 30–50 %, because of decreases in the size and number of tubers produced [2,3,4]
Some of the sampled plants showed characteristic PYVD symptoms in leaves to different degrees of intensity, as had been described before [1, 5], whereas others had atypical yellowing symptoms described as unevenly distributed green patches on yellow leaf laminas
Mixed viral infections involving PYVV appear to be very abundant in S. tuberosum fields in the Antioquia department of Colombia [24]
Summary
Potato yellow vein virus (PYVV) causes a disease known as potato yellow vein disease (PYVD) in potato crops in Colombia, Ecuador, Perú and Venezuela [1], with crop losses estimated at 30–50 %, because of decreases in the size and number of tubers produced [2,3,4]. In Colombia, two species of potato are cultivated commercially, Solanum phureja and S. tuberosum, the first a diploid species and second tetraploid [5]. The yield reductions are associated with the presence of symptoms, such as initial yellowing of the secondary and tertiary leaf-veins that later spreads throughout and around the foliar veins, covering the lamina. These bright yellow leaf symptoms cause a reduction in the photosynthetic capacity and vigour of the plants [3]. It has been reported that in the field, a proportion of the infected plants may not display symptoms [1, 7, 8] and that yields of such plants are not reduced [3]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
