Molecular insight reveals broad-scale spatial patterns in floodplain ciliate assemblages, whereas morphology reflects local environmental controls

Abstract

One of the major goals in microbial ecology is to understand whether the empirical biogeographic patterns of macroorganisms also apply to microorganisms. Here, we used morphological data from live organisms, along with molecular data, to investigate the importance of spatial factors and environmental variables in influencing ciliate composition from floodplain lakes. Our main goal was to use 2 different approaches (morphological and molecular) to compare ciliate diversity and distribution patterns as well as to compare how these methods differ in their ability to detect distribution patterns and the roles of spatial and environmental factors that shape ciliate assemblages in the 4 largest floodplains in Brazil. Planktonic water samples were gathered from 33 lakes associated with 4 different river floodplain systems in Brazil. We analyzed ciliates in vivo and sequenced surface water DNA using a metabarcoding approach with general eukaryotic primers. We showed that the diversity of operational taxonomic units was much higher than that of morphospecies. Regardless of the method of identification, we found a consistent spatial assembly pattern of ciliate assemblages across the 4 floodplain systems. We also found that environmental filters had a stronger association with the morphological than with the molecular site-by-site dissimilarities. Meanwhile, biogeographic factors and the distance among sites limited the distribution of molecular-based composition, resulting in strong differences among the floodplain lakes analyzed. This finding suggests that ecological research and biomonitoring activities should find an equilibrium between morphological and molecular approaches because each approach provides unique insights.