Molecular Identification of Trametes Species Collected from Ondo and Oyo States , Nigeria

Abstract

Internal Transcribed Spacer (ITS) region of the rDNA was used in the identification of Trametes species collected from Nigeria. Ribosomal DNA of Trametes species were extracted using CTAB lysis method. The extracted DNAs were amplified using ITS region and the amplicons sequenced. BLAST search on NCBI GenBank revealed that Trametes species from Nigeria are more related to T. lactinea, T. elegans, T. polyzona, T. cingulata and T. ijubarskii with percentage relationship of 96 to 99%. Phylogenetic tree generated from Trametes sequences from Nigeria and sequences obtained from NCBI GenBank revealed 7 clades, out of which Trametes from Nigeria were placed in 3 separate clades. This study showed that most of the gene sequences of Trametes species indigenous to Nigeria are not 100% homologous with existing gene sequence found in NCBI GenBank. The closest Trametes species to the Trametes species indigenous to Nigeria are T. lactinea and T. polyzona with 99% level of similarity.