Molecular Identification of Streptococcus equi subspecies equi in Horses

S H Mahmood

doi:10.30539/iraqijvm.v38i2.215

Abstract

The objective of this study to evaluate the existence of Streptococcus equi subspecies equi as probable agents of naturally occurring infection of the equine upper respiratory disease from the Equestrian club in Baghdad city. Nasal swabs and pus samples from 141 horses with upper respiratory tract infections were collected. Results indicated that different microorganisms were isolated and identified S. equi subsp equi (30 isolates), S. equi subsp zooepidemicus (14 isolates), S. equisimilus (9 isolates), Enterococcus. fecalis (17 isolates), Pasteurella spp. (29 isolates), Staphylococcus spp. (25 isolates), Bacillus spp. (24 isolates), Pseudomonas spp.(16 isolates), and E. coli (21 isolates). All 30 isolates of S. equi was characterized by biochemical tests. For molecular identification of the subspecies S. equi one genomic region SeM was amplified.

Highlights

Streptococcus equi is the causative agent Affected horses typically become anorexic, of strangles, a contagious inflammatory depressed, and develop bilateral, serous to disease of the upper respiratory tract the mucoid nasal discharge within 24 hours of outcome is only rarely fatal due to onset of fever
Samples were collected from nasal cavities of horses by using sterile swabs, pus swabs from open lymph node by using sterile swabs, and pus samples by using sterile disposable syringe which 18G from un-open lymph node, Samples were transported to the laboratory under sterile condition, where the required tests were done or stored at -20 ̊C
Serous and mucopurulent nasal discharge and ocular discharge, enlargement of the submandibular lymph node, some of it was un-open and some was open and excreted pus which was creamy in color, congestion of mucous membrane, and in one case there was abscess in neck and legs of horse

Summary

Introduction

Streptococcus equi is the causative agent Affected horses typically become anorexic, of strangles, a contagious inflammatory depressed, and develop bilateral, serous to disease of the upper respiratory tract the mucoid nasal discharge within 24 hours of outcome is only rarely fatal due to onset of fever. Strangles is a common mucopurulent as the disease progresses, and a infection among the horse population. It is a moist cough may develop in some cases. A routine laboratory 10 days after the onset of clinical signs and, in detection of the organism involving culture of uncomplicated cases; recovery is complete 1 to nasal swabs and pus from abscesses is often 2 weeks thereafter [9].

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