Molecular Identification of some isolates of Fungi Isolated from Al-Mishkhab Rice Field research station

Abstract

This study was conducted at the Microbiology Laboratory of the Department of Environmental Science - College of Science - University of Kufa with the aim of isolating and diagnosing fungi isolated from the rice field at the Rice Research Station in Al-Mishkhab in Najaf Governorate. Fungi isolates were diagnosed using the Polymerase Chain Reaction (PCR) technique and nucleotide sequence determination for DNA products multiplied using the ITS1 and ITS4 prefixes. The results of analyzing the nucleotide sequence of the double DNA products of fungal isolates isolated in this study and using the BLAST program showed that some fungal isolates in this study refer to Pencillium citrinum (1), others belong to Alternaria alternata (2), Aspergillus rugulosus (3), F. chlamydosporum (4). Trichoderma harzianum(5) showed a difference in the sequence of some nitrogenous bases for DNA products. Aspergillus flavus (6), Penicillium Spinulosum (7). The results showed that two isolates of fungi isolates isolated in this study (Aspergillus rugulosus and Aspergillus flavus) were not registered formerly at the National Center for Biotechnology Information (NCBI), so they were registered under the numbers MT356126 and MT358845.