Molecular identification of some allergenic fungi found in household dust in Mosul city

Abstract

The study included isolating and diagnosing the fungi found in dust samples from homes and buildings such as basements and walls containing fungal growth in separate areas from the left side of the city of Mosul in northern Iraq, such as Al-Ghufran neighborhood, Al-Mazare’ neighborhood, and Al-Mohandesin neighborhood during October and November, and the relationship of these fungi to human diseases, including allergies and asthma. The isolation results showed many fungal genera, including Cladosporium, Penicillium, Aspergillus, Alternaria and Trichoderma. The research aims to study the fungi Alternaria and Trichoderma, where the percentage of the presence of Alternaria in the wall sample containing the previous fungal growth was 28.57%. Whereas the percentage of the presence of Trichoderma fungus in dust and gypsum falling on the surfaces of poorly ventilated rooms in the cellars was 42.86%, and the molecular diagnosis of fungal isolates was carried out, as it was confirmed that there is a match with the standard strains found in the gene bank. The Alt a1 and Exp genes responsible for asthma were also examined and detected in fungal isolates using PCR technology and polymerase chain reaction; the new genes in both isolates were recorded. On behalf of both the supervisor and the researcher with international numbers in the global gene bank. Keywords: Household dust, allergies and asthma, indoor environments, Alternaria and Trichoderma, Mosul local fungi.