MOLECULAR IDENTIFICATION OF SHOREA JOHORENSIS IN KETAMBE RESEARCH STATION, GUNUNG LEUSER NATIONAL PARK

Nir Fathiya,Zairin Thomy,Iqbar Iqbar,Essy Harnelly

doi:10.24815/jn.v18i2.10123

Abstract

Shorea johorensis is one of the red meranti plants in Ketambe Research Station, Gunung Leuser National Park, Aceh Tenggara. Currently, Shorea johorensis also is well known as a major source of valuable commercial timber. This research aimed to analyze the phylogenetic of Shorea johorensis based on chloroplast and nuclear DNA in Ketambe Research Station so that it can be known the relationship of Shorea johorensis with other species Dipterocarpaceae in GenBank database. The research was conducted from July 2015 to August 2016 in Ketambe Research Station and Forestry and Forest Genetics Laboratory of Molecular, Bogor Agricultural University. The method used quadrat sampling technique with purposive sampling and experimental laboratory that consisted of DNA extraction, PCR, electrophoresis, and sequencing. The data analysis was done using BioEdit, MEGA6, and BLAST. The results showed that the phylogenetic tree of Shorea johorensis based on the rbcL and matK showed that Shorea johorensis was closely related with some species Hopea; but the phylogenetic tree based on psbA-trnH, 5.8S rRNA, ITS2, and 28S rRNA showed that Shorea johorensis was closely related with Shorea robusta.Keywords: Chloroplast dna, Ketambe Research Station,Nuclear DNA, Shorea johorensis

Highlights

Gunung Leuser National Park is one of Nature Conservation Areas in Indonesia covering an area of 1.094.692 hectares
A chloroplast DNA marker that is Molecular Identification of Shorea johorensis In Ketambe Research Station, Gunung ... (Nir Fathiya, Essy Harnelly, Zairin Thomy and Iqbar) maternally inherited shows more conserved DNA patterns compared with a nuclear gene that is biparentally inherited
This research aims to analyze the phylogenetic of Shorea johorensis based on chloroplast and nuclear DNA in Ketambe Research Station so that the relationship of Shorea johorensis with some species in Dipterocarpaceae can be determined

Summary

Introduction

Gunung Leuser National Park is one of Nature Conservation Areas in Indonesia covering an area of 1.094.692 hectares. This national park is located in Aceh and Sumatera Utara provinces. A few Dipterocarpaceae species is found growing in Ketambe Research Station [2]. One of the Dipterocarpaceae species is Shorea johorensis. It can be found in Southeast Asian in Borneo, the Peninsular of Thailand and Peninsular Malaysia, and Sumatra. A chloroplast DNA marker that is Molecular Identification of Shorea johorensis In Ketambe Research Station, Gunung ... Several regions of chloroplast genome are rbcL gene, matK gene, and psbA-trnH intergenic spacer. The psbA-trnH intergenic spacer is an evolutionary plastid region and employed as a phylogenetic marker [10]

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