Molecular Identification of Several Orchid Species Based on OPA10 and OPA18 RAPD Marker

Abstract

The purpose of this study was to determine the genetic characteristics of nine orchids plants collected by Biology Gardens of Mathematics and Science Faculty of UNY based on OPA10 and OPA18 markers, to detect their genetic relationship, and determining the exact RAPD markers for identification of intraspecies and interspecies orchids. The analysis was carried out by isolating DNA from the leaves of 9 species of orchids (Rhynchostylis retusa, Vanda tricolor, Dendrobium aphyllum, Dendrobium fimbriatum, Dendrobium moschatum, Dendrobium crumenatum, Dendrobium antennatum, Dendrobium anosmum Gigantea Alba and Dendrobium anosmum Gigantea) using TIANGEN DNA healing plant kit. Molecular identification was analysed based on the PCR-RAPD technique using OPA 10 and OPA18 primers. Electrophoregraph of PCR results are converted into binary data and analysed with NTSYSpc 2.02 software. The similarity index (kinship level) was analysed based on UPGMA dendrogram. The results of the analysis showed that there was genetic diversity in 9 orchid plants tested. Intra-species orchids show higher similarity than inter-species. Primer OPA 10, OPA18, and a combination of both shows polymorphism in intra-species orchids.