Molecular Identification of Pm4 Powdery Mildew Resistant Gene in Oat

Sylwia Magdalena Okoń,Tomasz Ociepa,Aleksandra Nucia

doi:10.15835/nbha46210904

Abstract

The selection of specific plants with desirable traits supported by molecular markers is one of the most important tools in modern breeding programs, which lead to reduce time and cost of selection. The aim of presented study was identification of dominant markers associated with Pm4 powdery mildew resistant gene in oat. To identify dominant silicoDArT markers for Pm4 gene, F2 mapping population ‘Av1860’ × ‘Fuchs’ were analyzed using DArTseq methodology. Among obtained 46 230 silicoDArT markers, 126 markers were high correlated with resistance to powdery mildew in oat conditioned by Pm4 gene. Among selected markers, 48 sequences have been chosen for potential conversion into specific STS markers. Finally, only 20 were suitable for primer design. As a result, 5 converted markers amplified expected products in resistant bulks, 3 of them segregated according to resistance in the whole population and shoved high correlation coefficient between marker and phenotype observation. Converted markers based on PCR could be used for identification of Pm4 gene in oat. Obtained results confirm the possibility of converting silicoDArT markers into PCR-based technique, which can be used in marker assisted selection (MAS).

Highlights

Powdery mildew caused by Blumeria graminis DC. f.sp.avenae Em
Obtained results confirm the possibility of converting silicoDArT markers into PCR-based technique, which can be used in marker assisted selection (MAS)
Materials and Methods Plant material The subject of the study was segregating population derived from cross between line ‘Av1860’ possessing Pm4 powdery mildew resistant gene with susceptible oat cultivar ‘Fuchs’. 157 individuals of F2 population were phenotyped based on host-pathogen test

Summary

Introduction

Powdery mildew caused by Blumeria graminis DC. f.sp.avenae Em. Marchal. is one of the most important foliar disease of cultivated oat is. Is one of the most important foliar disease of cultivated oat is This worldwide disease appears every year and causes big crop loses (Lawes and Hayes, 1965; Jones, 1977; Schwarzbach and Smith, 1988; Sebesta et al, 1991; Clifford, 1995; Aung et al, 1977; Roderick et al, 2000). The use of fungicides is the easiest method of protection against pathogen attack, but cultivated oat is a low-input crop which grown on less productive lands, so in this case, breeding resistant cultivars seems to be more economical and more environmental friendly method for controlling and limitation occurrence of oat powdery mildew. Nowadays the most effective sources against powdery mildew in oat is Pm4 resistant gene (Okoń, 2015)

Methods

Results

Conclusion