Abstract
Mycobacterium bovis (M. bovis) BCG belongs to Mycobacterium tuberculosis complex (MTBC), highly relatedorganisms, which are 99.9 % similar at nucleotide level and phenotypically similar. Its differentiation from other membersof MTBC by conventional methods is laborious and time consuming. PCR provides a rapid alternative method fordifferentiation between members of MTBC. It depends on identification of region of difference (RD) which have beenlost during attenuation of M. bovis. Two different BCG strains (from two sources) were confirmed as a member of M.tuberculosis (MTB) complex (MTC) and as BCG strains by PCR using primers to a region of the 16S rRNA gene that isconserved in all mycobacteria and region of difference (RD1, RD4, RD9 and RD12) respectively. DNA of Mycobacteriumtuberculosis was used as a control to compare with BCG strains. The results showed that 16S rRNA gene was present inall tested strains, while the RD1, RD4, RD9 and RD12 were absent only in BCG strains
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
