Abstract

In this study Streptomyces were isolated from 50 bacterial isolates taken from 30 soil samples, these samples were collected from various locations in Iraq's various regions. The species of Streptomyces were isolated using starch casein agar and diagnosed microscopically and morphologically by Gram staining and glass slide. The sequence analysis 16S rRNA is used to report 11 Streptomyces. 10 bands of DNA gene, a result of specific polymerase chain reaction PCR, are elected from bacterial local isolates where 1000 base pairs within one volume, The PCR products of DNA samples were chosen from 11 local isolates based on nitrogenous base sequences. These organisms are revealed as a result of the study and by using DNA Blast NCBI as fellows; Streptomyces gancidicus, S. werraensis, S. griseorubens, S. hawaiiensis, S. thermocarboxydus, S. cyaneus, S. misionensis, S. bellus, S. parvulus, S. labedae,

Highlights

  • The species of streptomyces are widely prevalent in soil, It is the largest genus of the Actinomycetes, Gram-positive bacteria

  • The higher rate of GC is ∼70% of the content of Streptomyces spp

  • This research aims to identify and isolate Streptomyces from the soil of northern Iraq (Nineveh, Duhok, Erbil) as well as diagnose them using microscopic and morphological experiments and a PCR test based on 16S rRNA to establish their genetic sequence

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Summary

Introduction

The species of streptomyces are widely prevalent in soil, It is the largest genus of the Actinomycetes, Gram-positive bacteria. Their colors mostly are grey but few are red, green, and white, while the blue is the rarest [1]. The genus Streptomyces is considered from Actinomycetes diameter (0.52) μm. It is grown Aerial mycelium carrying many spores, that are arranged in chains and take different shapes, from the spiral form, Rectus- flexibilis form, Retinaculum- Apertum form [2] [3]. A scented substance produced by Streptomyces is responsible for the distinctive odor of soil.

Collecting of Samples
Characteristics of Streptomyces
Streptomyces detection
The media
Nutrient agar medium
PCR Reactions
DNA Sequencing analysis
Findings
Genomic DNA polymer reaction
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