Abstract
Five DNA barcodes were tested for identification and discrimination of Lavendula dentata L., Mentha longifolia (L.) Huds. and Mentha × piperita L. New DNA barcodes have been registered for L. dentata from Taif, Saudi Arabia. The separate clading of L. dentata and M. longifolia through the phylogenic analyses proved their endemism to Saudi Arabia. The phylogenetic trees revealed from the ITS2, matK and trnH data demonstrated that all Mentha species formed monophyletic clusters except hybrid M. × piperita from Taif which formed separate clades distinguishing it from the two parents; M. aquatica L. and M. spicata L. DNA barcoding could be considered as a good approach for distinguishing and identifying the mint plants, though it was not possible to confirm the relationship between hybrids and their putative parents.
Highlights
The family Lamiaceae comprising about 7,173 species under 236 genera possesses medicinal and aromatic herbs such as lavender, basil, mint, rosemary and thyme, that have been widely utilized as teas, spices, traditional medicines or raw material for the food and pharmaceutical industries (Theodoridis et al, 2012).Lavendula dentata is one of five naturally growing lavender species in Saudi Arabia that has been known as the main center of origin of the genus (Miller, 1985)
Email: shamahmoh@gmail.com i) utility of specific DNA regions, two nuclear internal transcribed spacers (ITS and ITS2) and the plastid DNA regions for developing DNA barcodes and subsequently identification for the three species; Lavendula dentata, Mentha longifolia and M. × piperita occurred in Taif highlands of Saudi Arabia; ii) discriminating between species under study and those retrieved from the GenBank and iii) exploring the interspecific variation between M. longifolia and M. × piperita
Sequences of L. dentata for ITS, ITS2, rbcL and trnH barcoding loci were submitted to BLAST at the GenBank database, any sequence of L. dentata was detected in the database, the present study succeeded in registering new DNA barcodes for L. dentata from
Summary
The family Lamiaceae comprising about 7,173 species under 236 genera possesses medicinal and aromatic herbs such as lavender, basil, mint, rosemary and thyme, that have been widely utilized as teas, spices, traditional medicines or raw material for the food and pharmaceutical industries (Theodoridis et al, 2012).Lavendula dentata is one of five naturally growing lavender species in Saudi Arabia that has been known as the main center of origin of the genus (Miller, 1985). × piperita occurred in Taif highlands of Saudi Arabia; ii) discriminating between species under study and those retrieved from the GenBank and iii) exploring the interspecific variation between M. longifolia and M. Sequences alignment and phylogenetic analyses The sequences of ITS, ITS2, matK, rbcL and trnH of L. dentata, M. longifolia and M. MOLECULAR IDENTIFICATION OF LAVENDULA DENTATA, MENTHA LONGIFOLIA AND M.× PIPERITA 151
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