Molecular identification of fungal isolates from rhizospheric soil of medicinal plants

Abstract

Further knowledge of the interactions between rhizospheric fungi and medicinal plants is needed to promote the manufacture of crude medications, as it has recently been clear how these fungi have a significant impact on the quality and quantity of crude pharmaceuticals through a specific fungus-host interaction. The present study was aimed to find out the diversities of fungi in the rhizospheric soil of medicinal plants particularly (Gingiber officinale, Calotropis gigantea, Endographis paniculata, Vigna mungo, Chrysopogon giganoids and Tinospora cordifolia) collected from CIMAP CSIR situated in Indian city Lucknow and from nearby Paikramau agriculture field Lucknow, U.P., India. The DNA of the rhizospheric fungal isolates was amplified through PCR for species level identification using a specific internal transcribed spacer primer (ITS1/ITS4). This study revealed that five different type of fungi identified surrounding the Rhizospheric region of the selected aforementioned plants such as Rhizopus oryzae, Fusarium incarnatum, Penicillium expansum, Aspergillus ochraceous and Aspergillus flavus further validated by sequencing the PCR findings and comparing them to other relevant sequences in GenBank (NCBI).