Molecular identification of Enterobius vermicularis worms isolated from children in Babylon province of Iraq

Abstract

Background and Objective: The conventional PCR technique was using for study genetic variations for Cytochrome C oxidase subunit 1 (cox1) gene of E. vermicularis between local and global to NCBI-BLAST E. vermicularis. Materials and methods: Stool samples were collected from children at early morning then transported to the laboratory. The DNA cox1 gene were isolated from stool samples then amplified by PCR technique and its results were analysis via agarose gel electrophoresis. The DNA sequencing method was carried out by sent PCR products to Macrogen Company in Korea. Phylogenetic analysis was conducted by using (UPGMA tree) method in (MEGA 6.0 version). Results: Results appears that Stool samples was positive for infection with E. vermicularis worm, DNA for cox1 gene was amplified to 407 bp that was positive at agarose gel electrophoresis. The human E. vermicularis local isolates showed homology identity to the NCBI-BLAST E. vermicularis human isolates from Iran.The phylogenetic analysis was accomplished to constructing the phylogenetic tree. The sequences of local isolates were showed have genetic relationship to NCBI-BLAST E. vermicularis with total genetic variations (0.0080-0.0020). Conclusion: PCR technique is valuable technique for isolate and know genetic variations E. vermicularis COX1 DNA genes.