Abstract
Infection with Cystoisospora belli causes severe diarrhea, bile duct involvement, and Extra-intestinal spread in patients with acquired immunodeficiency syndrome (AIDS). This study included eight adult patients with AIDS and chronic diarrhea diagnosed stool cystosisporiasis (oocysts) and / or duodenal biopsies (asexual and sexual stages in epithelium). Identification was carried out at the molecular level using a nested-polymerase chain reaction (PCR) technique that amplifies a fragment of the ribosomal ribonucleic acid (RNA) small subunit gene, using DNA extracted from feces and biopsies. This work allowed us to make the diagnosis of cystosisporiasis through analysis coproparasitological, optimize the DNA extraction protocol from samples fecal and implement the nested -PCR technique for the diagnosis of C. belli in biological samples from infected patients.
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