Abstract
Chickpea plants showing stunting, shoot proliferation and phyllody indicative of phytoplasma infection were observed in farmers’ fields at Pune, Maharashtra, India. The presence of phytoplasmas in all symptomatic samples was confirmed by PCR and nested PCR assay using phytoplasma-specific primer pairs P1/P7 and R16F2n/R2. Sequence analysis showed that the amplicon sequence shares more than 99% identity with sequences of ‘Candidatus Phytoplasma aurantifolia’ and in the phylogenetic analysis, the sequence clustered with other phytoplasma sequences belonging to this ‘Ca. Phytoplasma’ species. Virtual RFLP analysis of the sequence enabled its assignment to 16SrII-C subgroup. This is the first report of 16SrII-C phytoplasmas associated with chickpea phyllody from western Maharashtra.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
