Abstract
This study was carried out for molecular identification and sexual differentiation of freshwater mud eel, Monopterus cuchia which is most important for induced breeding. Traditional classification of freshwater eels has always been obscurerd and unreliable due to their morphological ambiguity. A rapid and cost effective molecular markers, mitochondrial 16S rRNA and glutamine synthetase gene was used to establish molecular standards for identification of this fish. Similar bands were seen in all the individuals at the level of 250bp length by using 16s mitochondrial DNA of 24 individuals and 544bp length for partial sequence of glutamine synthetage gene in 11 individuals. A successful protocol was developed to identify male and female M. cuchia through morphological, anatomical and histological analysis.
Highlights
Monopterus cuchia [1] is commonly found in open water, mud holes, haors, canals, beels, paddy fields and floodplains with natural care of different districts of Bangladesh [2]
PCR reaction was done for both the primers with 25μl of master mix for each sample. In this experiment PCR reaction of 16s mitochondrial DNA was conducted by 40 cycles with preheated at 94oC for 3 minutes followed by denaturation at 94oC for 1 minute, annealing at 58oc for 1 minute and 2 minutes for extension at 72oC
Identification of M. cuchia needs to be supported by molecular characterization instead of conventional methods [16]
Summary
Monopterus cuchia [1] is commonly found in open water, mud holes, haors, canals, beels, paddy fields and floodplains with natural care of different districts of Bangladesh [2]. With ecological importance and high nutritional components this fish can play a unique role for the development of socio-economic status of fishermen as well as with short of culture practice [3]. Due to its high economic, ecological, nutritional and medicinal values, the research on this fish is most appropriate to enhance the stock in nature in terms of its conservation, breeding, culture and production. Since the external morphology of Japanese eel, European eel, and American eel is quite similar to each other as well as different Monopterus species, it is necessary to apply a more sensitive and rapid method for species identification. Appropriate identification of this fish is essential for the proper development of this fish
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