Abstract
Conventional taxonomy is limited with delineating species and controversies arise with DNA barcoding based identifications. Hence, an alternative supporting approach is very much needed to identify species and differentiate them within the species based on the genetic material. 18S rRNA genes have been particularly helpful in analyzing phylogeny at the species level. In addition, bioinformatics which represents a new, growing area of science uses computational approaches to answer biological questions. Salt tolerant costal salt marsh plant of Suaeda maritima was selected for 18s rRNA sequencing to solve the ambiguity in itsspecies level identification. Similarity search of study species shared 99% similarity with 5 species of Atriplex canescens clone s128, Atriplex torreyi var. griffithsii clone p508, Spinacia oleracea, Oenothera laciniata clone,Beta vulgaris. Phylogenetic tree infer that S.maritima is closely related to Spinacia oleracea and Oenothera laciniata. Atriplex canescens (fourwing saltbush), Atriplex torreyi and Phaulothamnus spinescens, Celosia argentea found to be closely related and are in one group. Hence, this study result clearly shows thus study species evaluated from angiosperm and provides key step in understanding the evolution of salt tolerance in angiosperm.
Highlights
Coastal habitats represent important ecosystems in terms of biodiversity; they can be broadly classified into four distinct types: salt marshes and mangroves, sand dunes, vegetated shingle and rocky cliffs
An alternative supporting approach is very much needed to identify species and differentiate them within the species based on the genetic material. 18S rRNA genes have been helpful in analyzing phylogeny at the species level
Salt tolerant costal salt marsh plant of Suaeda maritima was selected for 18s rRNA sequencing to solve the ambiguity in its species level identification
Summary
Coastal habitats represent important ecosystems in terms of biodiversity; they can be broadly classified into four distinct types: salt marshes and mangroves, sand dunes, vegetated shingle and rocky cliffs. The plants that grow on coastal marshes must be tolerant to the varying saline conditions. Halophytic plants are generally located in coastal areas and they exist as in land salt marshes in appropriate geological and climatological settings around the globe. Halophytes represent salttolerant species that thrive in the inhospitable habitats of inland and coastal salt marshes, dunes, beaches, deserts and salt flats (Flowers et al, 1986, W.H.O, 1990). Different physiological and anatomical traits of halophytes vary between species to survive in saline habitats. The morpho-anatomical alterations of succulent halophytes include increase of cell volume, especially of spongy and water paenchma increase of leaf thickness and decrease in number of stomata (Strogonov, 1973)
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