Abstract
The aim of this study was to identify the genetic diversity of lactic acid bacteria (LAB) isolated from the local goat's milk. A total of 100 raw milk samples were collected from the different Basrah local markets. All the samples were cultured in the De man, Rogosa, and Sharpe (MRS) medium which enhances the growth of lactic acid bacteria. The result of the study showed that the only 64 lactic acid bacteria isolated gave the Gram-positive and catalase-negative were 64 (64%). All the suspected isolates were detected and identified by using polymerase chain reaction (PCR) targeting the 16S rRNA gene and DNA sequencing. The sequencing results showed that 9 strains belong to Lactococcus spp. and 6 strainsbelongto Lactobacillus spp. and all tested isolates had similarity over 99% with those recorded in the GenBank of The National Centre for Biotechnology.
Highlights
Over several centuries, Lactic Acid Bacteria (LAB) have an essential role in the manufacture and preservation of many fermented food products
The LAB are widely distributed in nature, they are found in the environments where carbohydrates are available, such as food, respiratory, gastrointestinal tract (GIT), genital tracts of humans and animals, in sewage, and the plant material [13]
Isolation of lactic acid bacteria The total number of lactic acid bacteria isolated from goat's milk were 64 isolates
Summary
Lactic Acid Bacteria (LAB) have an essential role in the manufacture and preservation of many fermented food products. Many of the previous investigations showed that the lactobacilli have significant effects on the prevention and treatment of various human gastrointestinal disorders, infectious enterocolitis, besides enteric and colorectal cancers [7,8,9]. In domestic ruminants, these bacteria play an important role in improving nutritional efficiency [10,11,12]. Goat milk has gained interest mainly because of its iron bioavailability, the higher concentration of fatty acids and the lower allergenicity [16]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
