Abstract
The current study aimed to identify the selected number of Campylobacter strains of poultry origin in India that were isolated in the Laboratory of International Prevention of Epidemics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan to the species level with the aid of cdtA/B/C gene-based multiplex PCR assays as well as to investigate their antimicrobial resistance profiles. C. jejuni (4 strains) and C. coli (16 strains) were identified. The poultry isolates identified were subjected to susceptibility testing with the aid of disk diffusion method using 12 antimicrobial agents. Again, the resistant and intermediate isolates confirmed by the disk diffusion method were subjected to determination of minimum inhibitory concentration by agar dilution method. To elucidate the mechanism of quinolone resistance, a total number of 20 quinolone resistant strains were subjected to sequence determination and analysis of the gyrA gene in the quinolone-resistance determining region. The results of the disk diffusion method were consistent with the results of the agar dilution method with slight variation in case of ampicillin, levofloxacin, ofloxacin and fosfomycin. All C. jejuni and C. coli strains had the Thr 86 to Ile substitution in gyrA. Results of this study support the emergence of resistance of C. jejuni and C. coli strains of poultry origin in India to a variety of antimicrobials especially fluoroquinolones.Asian J. Med. Biol. Res. June 2015, 1(1): 1-8
Highlights
Campylobacter, a gram negative, nonsporulating, motile bacterium, is commonly isolated as a pathogen associated with diarrhoea in many industrialized countries (Stanley and Jones, 2003)
Among the 20 Campylobacter strains isolated from poultry origin in India, 4 were identified as C. jejuni and the rest 16 were identified as C. coli with the aid of hipO gene based PCR, 16S rRNA gene sequencing and cdtA/B/C gene based multiplex PCR assays
Detection of C. jejuni and C. coli by cdtB gene-based multiplex PCR are shown in Figures 1 and 2
Summary
Campylobacter, a gram negative, nonsporulating, motile bacterium, is commonly isolated as a pathogen associated with diarrhoea in many industrialized countries (Stanley and Jones, 2003). Studies on isolation of Campylobacter from poultry meat have been carried out from the regions Tamilnadu and Calcutta using conventional method (Chowdhury et al, 1984; Varma et al, 2000). Campylobacter infections pose a serious public health problem for which many countries are monitoring their infection and antimicrobial resistance patterns. The current study was aimed to identify the selected number of Campylobacter strains of poultry origin in Calcutta, India that were isolated in the Laboratory of International Prevention of Epidemics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Japan to the species level with the aid of cdtA/B/C gene-based multiplex PCR assays as well as to investigate their antimicrobial resistance profiles
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