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Abstract
ObjectiveTo determine the prevalence and distribution of human enteroviruses (HEVs) among healthy children in Shenzhen, China.MethodClinical specimens were obtained from 320 healthy children under 5 years old in Shenzhen, China from 2010 to 2011. The specimens were evaluated using real-time PCR and cell cultures. The positive specimens were further tested using reverse transcription-seminested PCR (RT-snPCR). Molecular typing and phylogenetic analysis were based on the sequence determined.ResultsAmong the 320 samples, 34 were tested positive for HEVs (10.6%) and 22 different serotypes were identified using RT-snPCR. PV1 and PV2 were also detected. The predominant serotype observed was EV71 (17.6%), followed by CV-B4 (14.7%). HEV-B was detected most frequently, with an overall prevalence of 47.1%. HEV-A and HEV-C were found in 32.3% and 20.6% of the samples, respectively. No HEV-D was identified. Molecular phylogeny indicated that all EV71 strains were of C4 genotype.ConclusionAlthough a variety of HEVs was detected in healthy children, HEV-B was relatively more prevalent than other HEV species. Considering HEV-A is more prevalent than HEV-B among patients with hand-foot-mouth disease, additional long-term surveillance of HEV is warranted in both asymptomatic and symptomatic populations.
Highlights
Enteroviruses are small, single-stranded, positive-sense RNA viruses from the genus Enterovirus of family Picornaviridae
Conclusion: a variety of human enteroviruses (HEVs) was detected in healthy children, Human enterovirus B (HEV-B) was relatively more prevalent than other HEV species
Considering Human enterovirus A (HEV-A) is more prevalent than HEV-B among patients with hand-foot-mouth disease, additional long-term surveillance of HEV is warranted in both asymptomatic and symptomatic populations
Summary
Enteroviruses are small, single-stranded, positive-sense RNA viruses from the genus Enterovirus of family Picornaviridae. Since 1970, the original classification for human enteroviruses has been modified based on the molecular and biological properties. This revised classification recognizes at least 100 subtypes, classified into four species: Human enterovirus A (HEV-A) species include EV71, CVA16, CVA2-8, CVA10, CVA12, CVA16 and EV89-92; HEVB species include CVA9, CVB1-6, most echoviruses, and some new enteroviruses; HEV-C species consist of PV1-3, the majority of coxsackievirus A, and some new enteroviruses; HEV-D species only include EV68, EV70 and EV94 [4]. Enteroviruses cause illness in humans at any age and children ,5 years old are the most susceptible
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