Molecular Identifcation of 16S rRNA gene in Staphylococcus aureus Isolated from Wounds and Burns by PCR Technique and Study Resistance of Fusidic acid

Abstract

Thirty six samples were collected from patient suffering from wounds and burns in Baghdad Hospitals .The samples were analyzed for the presence ofStaphylococcus aureus. Ten positive samples screened by phenotypic microbiological and Biochemical tests and genotypic by DNA extracted from all isolates and the PCR carried out using 16S rRNA gene for S. aureus. In this method for identifying and confrmed all the staphylococcal isolates as S. aureus.The present study was carried out in an attempt to detect the distribution of antibiotic-resistant and we have tried to cover the ever increasing problems facing the treatment and containment of bacterial skin infections.Therefore we are studySusceptibility of Staphylococcus aureus to antibiotics .The results showed relatively high resistance to MethcillinME ,Amoxicillin-Clavilonicacid, Azithromycin with frequencies of 100%, 100% and 70% respectively and moderate resistance to Vancomycin VA, Amikacin AK with frequencies of 60% and 62% respectively, low resistance to Fusidic acid with frequencies of 30% because that we determined the Minimum Inhibitory Concentration for Fusidic acid ,the isolates incorporated in this test were those have intermediate resistant to Fusidic acid.