Abstract
Mancini et al. (1965) have reported a method for immunological quantitation of proteins by means of single radial diffusion, which is now used extensively in immunochemistry for measurement of immunoglobulin concentrations in sera. Using this method, in instances of monospecific antibodies, to quantitate immunoglobulins leads in some cases to difficulties because of multiple ring precipitations. In this communication we report multiple precipitation in single radial diffusion, using recently commercially available plates (Tri-partigen plates, Behringwerke, Marburg, Germany), as an indication of molecular heterogeneity in different immunoglobulin classes.
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