Molecular-genetic features of MSSA, isolated in Kazakhstan

Abstract

Background: S. aureus colonization in the community and medical facilities may contribute to the extension of such diseases as the surface skin and soft tissue infections and life-threatening diseases–sepsis, osteomyelitis, endocarditis and toxic shock syndrome. S. aureus carriage is a major risk factor for the subsequent development of nosocomial and community-acquired infections. Knowledge of the prevalence of certain MSSA clones can be used to predict the spread of MSSA and MRSA infections. Objective: To study the molecular-genetic features of MSSA, isolated in Kazakhstan Material/methods: The identification of 51 community-acquired S. aures strains performed by MALDI-TOF MS (Bruker Microflex, Biotyper software V. 3.1.66 and database v 3.2.1.1, Bruker Daltonics, Germany). MSSA confirmed phenotypically using cefoxitin. Amplification of SCCmec was conducted according to the procedure Chongtrakool et al. (2006). Spa-typing was conducted to confirm the clonal relatedness (Harmsen, 2003). Sequencing was carried out in aautomatic capillary system ABI3500 (Applied Biosystems), spa gene sequencing results processed by Ridom program (Ridom) and Sequencing analysis (Applied Biosystems). Results: MSSA strains were characterized by a lack of SCCmecA cassettes. The dominant MSSA spa-types were t002 and t008. The study of clonality based on the clustering of the frequency of genetic events by eBURST method showed no clear clonal structure and regional specificities. Conclusion: The clustering did not reveal the clonality in distributing of spa-types data during the identifying of dominant spa-types. This fact may indicate the absence of epidemiological connection between the studied strains.