Abstract
Two Egyptian goat breeds (Baladi and Barki) were characterized using biochemical (SDS-PAGE and Native-PAGE) and molecular genetics (RAPD-PCR) techniques. Ten individuals from each breed were blood sampled. SDS-protein profiles for Barki breed showed lower percentage of similarity (82%) than Baladi breed (91%) within each one of them, while Native-PAGE analysis showed a high homogeneity value within each of the studied goat breeds. Moreover, the mean of the band frequencies averages within Baladi and Barki goat breeds based on SDS-PAGE were 0.88 and 0.77, respectively. RAPD analysis using 10 random primers showed high similarity average values of each breed for all used primers which were 0.85 and 0.83 for Baladi and Barki breeds, respectively, which indicates the presence of low genetic variations within the studied breeds. However, the similarity average value of the two tested breeds for all used primers was 0.84, which also indicating the presence of low genetic variations within the studied breeds.
Highlights
Two Egyptian goat breeds (Baladi and Barki) were characterized using biochemical (SDS-PAGE and Native-PAGE) and molecular genetics (RAPD-PCR) techniques
SDS-protein of ten blood plasma samples of each breed was used to assess the genetic similarity within the studied breeds (Baladi and Barki)
The similarity average values of each breed for all used primers were 0.85 and 0.83 for Baladi and Barki breeds, respectively, which indicates the presence of low genetic variations within the studied breeds
Summary
Two Egyptian goat breeds (Baladi and Barki) were characterized using biochemical (SDS-PAGE and Native-PAGE) and molecular genetics (RAPD-PCR) techniques. SDS-protein profiles for Barki breed showed lower percentage of similarity (82%) than Baladi breed (91%) within each one of them, while Native-PAGE analysis showed a high homogeneity value within each of the studied goat breeds. The mean of the band frequencies averages within Baladi and Barki goat breeds based on SDS-PAGE were 0.88 and 0.77, respectively. RAPD analysis using 10 random primers showed high similarity average values of each breed for all used primers which were 0.85 and 0.83 for Baladi and Barki breeds, respectively, which indicates the presence of low genetic variations within the studied breeds. The similarity average value of the two tested breeds for all used primers was 0.84, which indicating the presence of low genetic variations within the studied breed
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