Abstract
Real-time PCR was used to determine the ratio of viral and host DNA in lysates of Vero cells infected with HSV-1 strain L2. The number of virus copies reached a maximum after 24 h of incubation. Total isolated DNA was sequenced using the massively parallel sequencing technique on an Ion Torrent apparatus. Nucleotide sequences of thymidine kinase (UL23) and DNA polymerase (UL30) genes of a HSV-1 L2 population were determined; their primary structures were compared to those of other standard HSV-1 strains, KOS and 17. The detected differences between the UL23 and UL30 sequences of L2 and reference strains KOS and 17 were unimportant because these substitutions did not affect the conserved gene regions.
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