Molecular Exploring of Plasmid-mediated Ampc beta Lactamase Gene in Clinical Isolates of Proteus mirabilis

Abstract

Between September to December 2020, thirteen isolates of Proteus mirabilis were recovered among one hundred fifty; MacConkey agar was utilized to purify Gram-negative bacteria isolated from infections of the urinary tract. The primary identification of Proteus mirabilis isolates was relied on “colonial morphology, microscopic examination, and biochemical “tests; however, the confirmation of identification of antimicrobial susceptibility of isolates was conducted utilizing an automated VITEK-2 compact system. The result showed that Proteus mirabilis isolates were highly resistant to most antibiotics, making them multi-drug resistant (MDR). Phenotype methods were used to detect AmpC beta-lactamase. Initial and confirmatory methods showed that eight isolates were AmpC producers. Polymerase Chain Reaction (PCR) was employed to detect the blaampC gene.