Abstract
The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma, 18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed. Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases. An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio = 4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients.
Highlights
Infection with a high-risk human papillomavirus (HRHPV) type is necessary for the development of cervical carcinoma (Muñoz 2000)
An association between histological diagnosis of adenocarcinoma and HPV infection was observed (OR = 4.85, 95% confidence intervals (CIs) = 1.40-16.80, p = 0.009)
Our study showed that oncogenic HPV types can be detected in colorectal adenocarcinoma tissues from Cuban patients
Summary
Infection with a high-risk human papillomavirus (HRHPV) type is necessary for the development of cervical carcinoma (Muñoz 2000). Over the last few years, a possible correlation between HPV infection and colorectal cancer (CRC) has been suggested, based on several researchers detecting HPV DNA in these cancers, using different techniques (Buyru et al 2006, Damin et al 2007, Pérez et al 2010, Chen et al 2012). Findings of the present study indicate the presence of HPV DNA in colorectal tumours and suggest a role for oncogenic HPV types in the establishment of colorectal adenocarcinomas. These results may contribute to an understanding of the aetiology of sporadic CRC. To explore the possible relationship between HPV infection and colorectal carcinogenesis, we investigated the presence of HPV DNA in CRC tissues from Cuban patients and examined the relationship between HRHPV genotypes and viral loads and adenocarcinomas
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