Abstract
Abstract In this study, we sequenced two nuclear genes and one chloroplast spacer in Melastoma intermedium , a shrub species endemic to China, and its putative parental species, Melastoma candidum and Melastoma dodecandrum , to test the hybrid-origin hypothesis. Our results revealed that in one nuclear gene there were five fixed nucleotide substitutions between M. candidum and M. dodecandrum , and in the other nuclear gene, there were six. All but one individual of M. intermedium showed additivity in chromatograms at these sites of at least one gene. Haplotypes of M. candidum and M. dodecandrum at the two nuclear genes were well separated, and most haplotypes of M. intermedium were shared with those of M. candidum and M. dodecandrum . M. candidum and M. dodecandrum differed by three nucleotide substitutions in the chloroplast spacer, whereas individuals of M. intermedium had identical sequences to either M. candidum or M. dodecandrum. The molecular data clearly demonstrate that M. intermedium is of hybrid origin.
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