Abstract
Objective To investigate the molecular epidemiologic features of rabies outbreak in Chuxiong District in Yunnan Province,China,in 2011.Methods Brain tissue specimens from dogs and domestic animals were collected and the presence of rabies virus was determined by direct immunofluorescence (DFA) and reverse transcription-polymerase chain reaction (RT-PCR) assays.Homology and phylogenetic analyses were performed using the MegAlign and MEGA3.1software,respectively.Results Eleven of 23 brain tissue specimens were confirmed positive for rabies virus by both DFA and RT-PCR assays.A nucleotide sequence homology of 99.2％ -99.4％ existed between the two nucleoprotein ( N ) gene fragments from positive specimens and the Asia 1a subgroup of genotype 1Lyssavirus.Conclusion Phylogenetic analysis indicated that the rabies viruses causing this outbreak belonged to the Asia 1a subgroup of genotype 1Lyssavirus,which is closely related to the Yunnan-ZT07 specimen isolated in Zhaotong District,Yunnan Province,in 2007. Key words: Rabies virus; Epidemiology; Phylogeny
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