Abstract
BackgroundRotavirus A (RVA) and adenovirus (Adv) are important causes of acute diarrhea in children. RVAs are classified into G and P genotypes based on viral proteins (VP)7 and VP4 gene and Adv contains over 70 genotypes based on hexon and fiber gene. This study aimed to characterize the molecular epidemiology of RVA and Adv in children with acute diarrhea during 2017–2018 in Hangzhou.MethodsThe stool samples were collected and tested for RVA and Adv by reverse transcription-quantitative PCR (RT-qPCR) assay. The RVA positive samples were detected by RT-PCR for VP7(G) and VP4([P]) genotypes, and the Adv positive samples were detected by PCR for genotyping by the target to hexon gene.ResultsAmong 228 RVA-positive samples, G9 was detected as the most frequent genotype (195/228, 85.5%), followed by G3 (20/228, 8.8%), G2 (7/228, 3.1%) and G1 (6/228, 2.6%). G9 strains were closely related to strains from China and neighboring countries, as well as the USA. On the other hand, P[8] strains were detected in 219 (96.1%) samples with most closely related to one strain from Malawi, and P[4] in 9 (3.9%) samples. G9P[8] (84.6%, 193/228) was the most prevalent rotavirus A strains, followed by G3P[8] (8.8%, 20/228), G2P[4] (3.1%, 7/228), G1P[8] (2.6%, 6/228) and G9P[4] (0.9%, 2/228). Of 167 Adv-positive cases, 2 different genotypes were identified with 152 (91.0%) of Adv-41and 15 (9%) of Adv-40. All Adv strains were closely related to prototype strains of Adv types 40 and 41 in India.ConclusionsG9P[8] of RVA and Adv-41 were the most common genotypes that caused children’s acute diarrhea in Hangzhou, 2017–2018.
Highlights
Acute diarrhea is most commonly seen disease in young children with gastroenterological disorder and dehydration
Rotavirus A (RVA) are non-enveloped, and double-stranded RNA viruses that belong to the Reoviridae family
Template of one step reverse transcription-quantitative PCR (RT-qPCR) positive sample was used for further genotyping of RVA or Adv
Summary
Acute diarrhea is most commonly seen disease in young children with gastroenterological disorder and dehydration. Rotavirus A (RVA) and adenovirus (Adv) are the main viruses that cause acute diarrhea in children [1]. Li et al Gut Pathog (2020) 12:19 species (Adv A–G) have been characterized and classified phylogenetically according to the nucleic acid characteristics and homology as well as their hexon and fiber protein characteristics [5]. Among these species, Adv types, especially Adv-40 and -41 were found to frequently cause diarrhea in children [6]. Rotavirus A (RVA) and adenovirus (Adv) are important causes of acute diarrhea in children. This study aimed to characterize the molecular epidemiology of RVA and Adv in children with acute diarrhea during 2017–2018 in Hangzhou
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