Abstract
Resistant Pseudomonas aeruginosa isolates are one of the major causes of both hospital-acquired infections (HAIs) and community-acquired infections (CAIs). However, management of P. aeruginosa infections is difficult as the bacterium is inherently resistant to many antibiotics. In this study, a collection of 75 P. aeruginosa clinical isolates from two tertiary hospitals from Athens and Alexnadroupolis in Greece was studied to assess antimicrobial sensitivity and molecular epidemiology. All P. aeruginosa isolates were tested for susceptibility to 11 commonly used antibiotics, and the newly introduced Double Locus Sequence Typing (DLST) scheme was implemented to elucidate the predominant clones. The tested P. aeruginosa isolates presented various resistant phenotypes, with Verona Integron-Mediated Metallo-β-lactamase (VIM-2) mechanisms being the majority, and a new phenotype, FEPR-CAZS, being reported for the first time in Greek isolates. DLST revealed two predominant types, 32-39 and 8-37, and provided evidence for intra-hospital transmission of the 32-39 clone in one of the hospitals. The results indicate that DLST can be a valuable tool when local outbreaks demand immediate tracking investigation with limited time and financial resources.
Highlights
Resistant Pseudomonas aeruginosa isolates are one of the major causes of hospital-acquired infections (HAIs) and community-acquired infections (CAIs)
In H1, all 24 P. aeruginosa isolates were recovered from blood cultures of patients with a mean age of 65.41 ± 19.18 years and 12 (50%) males
Carbapenems are the most widespread antibiotics used in clinical practice to treat bacterial infections [38,39,40], and the resistance of P. aeruginosa isolates to these antibiotics is always a serious problem for the clinician
Summary
Resistant Pseudomonas aeruginosa isolates are one of the major causes of hospital-acquired infections (HAIs) and community-acquired infections (CAIs). CAIs are transmitted and develop outside the hospital, but demand hospitalization (e.g., pneumococcal pneumonia), or are clinically present within 48 h from hospital admission, regardless of the initial cause of hospitalization (e.g. chickenpox) [3]. Management of P. aeruginosa infections still remains a clinical challenge as the bacterium is inherently resistant to many antibiotics [4]. Due to the wide dispersion of the bacterium in the environment [4,7], as well as in the endogenous flora of hospitalized patients, it is important to implement powerful molecular typing tools to elucidate its molecular epidemiology and assess dispersal patterns of resistant strains [4]
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