Molecular epidemiology of Japanese encephalitis virus in mosquitoes in Taiwan during 2005-2012.

Chien-Ling Su,Pei-Yun Shu,Hwa-Jen Teng,Cheng-Fen Yang,Yu-Yu Chen,Li-Yu Chen,Kun-Hsien Tsai,Cheo Lin,Liang-Chen Lu,Shu-Fen Chang,Patricia V Aguilar

doi:10.1371/journal.pntd.0003122

Abstract

Japanese encephalitis (JE) is a mosquito-borne zoonotic disease caused by the Japanese encephalitis virus (JEV). Pigs and water birds are the main amplifying and maintenance hosts of the virus. In this study, we conducted a JEV survey in mosquitoes captured in pig farms and water bird wetland habitats in Taiwan during 2005 to 2012. A total of 102,633 mosquitoes were collected. Culex tritaeniorhynchus was the most common mosquito species found in the pig farms and wetlands. Among the 26 mosquito species collected, 11 tested positive for JEV by RT-PCR, including Cx. tritaeniorhynchus, Cx. annulus, Anopheles sinensis, Armigeres subalbatus, and Cx. fuscocephala. Among those testing positive, Cx. tritaeniorhynchus was the predominant vector species for the transmission of JEV genotypes I and III in Taiwan. The JEV infection rate was significantly higher in the mosquitoes from the pig farms than those from the wetlands. A phylogenetic analysis of the JEV envelope gene sequences isolated from the captured mosquitoes demonstrated that the predominant JEV genotype has shifted from genotype III to genotype I (GI), providing evidence for transmission cycle maintenance and multiple introductions of the GI strains in Taiwan during 2008 to 2012. This study demonstrates the intense JEV transmission activity in Taiwan, highlights the importance of JE vaccination for controlling the epidemic, and provides valuable information for the assessment of the vaccine's efficacy.

Highlights

Japanese encephalitis (JE) is a vector-borne zoonotic disease transmitted by the bite of a Japanese encephalitis virus (JEV) infected mosquito
Before 2008, all known JEV isolates collected in Taiwan belonged to Genotype III of JEV
The JEV infection rate of the mosquitoes captured on the pig farms was significantly higher than the rate of those captured in the wetlands, indicating that pigs played an important role in amplifying JEV

Summary

Introduction

Japanese encephalitis is a vector-borne zoonotic disease transmitted by the bite of a JEV-infected mosquito. JE is a vaccine preventable disease, JEV infections are still the leading cause of viral encephalitis in Asia [1], [2]. It is estimated that 67,900 JE cases occur annually in JE endemic countries, with an incidence rate of 1.8 cases per 100,000 individuals [3]. The mortality rate can reach as high as 30%, and approximately 30–50% of survivors suffer severe neurological damage [4], [5]. Recent studies suggested that the epidemic/dominant genotype of JEV has gradually shifted from genotype III (GIII) to genotype I (GI) in Southeast and East Asian countries in the last two decades [10,11,12,13,14]

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Discussion

Conclusion