Abstract
BackgroundHepatitis B virus (HBV) is highly contagious and causes liver diseases. Globally more than 350 million people are chronically infected and among them above 80 % are from developing countries like Bangladesh. Resistance to existing drugs and vaccines are common phenomenon due to mutations in HBsAg ‘a’ determinant. Due to lack of data about mutations and subtypes of HBV in Bangladesh, this study strongly demands to be documented. Here, we determined the genotypes and subtypes of HBV prevalent in Bangladesh, and their genomic mutations associated with vaccine and drug resistance.ResultsAmong 385 samples, a total of 54 (14 %) were found HBV positive, of which 19 samples were subjected to be sequenced. After bioinformatic analysis, we found Genotype D as predominant genotype (73.7 %) with subtypes ayw3 (64.3 %) and ayw2 (35.7 %), followed by genotype A with subtype adw2 (15.8 %), and then genotype C with subtype adr (10.5 %). A significant number of mutations (Thr118Val, Thr125Met, Thr126Ile, Pro127Thr, Ala128Val, Thr131Asn/Ser, Thr/Ser143Leu/Met) were found in ‘a’ determinant region which may admit resistance to the available vaccines and failure of HBsAg detection.ConclusionsThis comprehensive study have clinical importance like disease diagnosis and treatment. It emphasizes HBV infected patients to do molecular diagnosis for choice of anti-viral drugs and effectiveness of vaccines for proper treatment.
Highlights
Hepatitis B virus (HBV) is highly contagious and causes liver diseases
Among them 19 samples were randomly selected to be analyzed by polymerase chain reaction (PCR) and nucleotide sequencing
The purpose of this study was to determine the genotypes and subtypes of HBV prevailing in Bangladesh and to demonstrate possible mutations in ‘a’ determinant region that might be responsible for resistance to different anti-viral drugs and vaccines
Summary
Hepatitis B virus (HBV) is highly contagious and causes liver diseases. Globally more than 350 million people are chronically infected and among them above 80 % are from developing countries like Bangladesh. We determined the genotypes and subtypes of HBV prevalent in Bangladesh, and their genomic mutations associated with vaccine and drug resistance. Hepatitis B virus (HBV) infects over 350 million people globally per year and causes serious acute and chronic liver diseases (Mast et al 2005; Norder et al 1993, 1994). Genotyping and Subtyping of HBV is totally based on increasing of variety of antibodies to the ‘a’ determinant (Moradi et al 2012). Mutations in this region causes vaccine escape as well as HBsAg detection failure mutants through alteration of antigenicity of the protein (Carman et al 1990)
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