Molecular epidemiology of Aeromonas hydrophila based on 16 S ribosomal RNA and outer membrane protein W in different aquatic organisms

Abstract

Event Abstract Back to Event Molecular epidemiology of Aeromonas hydrophila based on 16 S ribosomal RNA and outer membrane protein W in different aquatic organisms Saurabh Dubey1, Kizito K. Mugimba2, Stephen Mutoloki1, Oystein Evensen1 and Hetron M. Munang'Andu1* 1 Norwegian University of Life Sciences, Norway 2 Makerere University, Faculty of Veterinary, Uganda Aeromonas hydrophila is one of the important pathogens that causes high mortalities in different freshwater fish species in aquaculture. Antigenic differences found on the surface proteins of A. hydrophila have led to characterization of this bacterial species into different serotypes in different fish species. To reduce the cost of producing different vaccines for different serotypes, this has led to the search of immunogenic proteins that are cross protective across different serotypes for use in vaccine development. Therefore, it is important that all A. hydrophila isolates from different aquatic organisms are correctly identified to facilitate the identification of immunogenic proteins with cross protective abilities across different serotypes. Recent developments have shown that the 16S ribosomal RNA (16S rRNA) is a useful molecular marker for the identification of different bacterial species. The 16S rRNA is ubiquitous to all members of the Enterobariaceae species that include A. hydrophila. In this study, we used the 16S rRNA to compare the genetic characteristics of A. hydrophila isolates from different aquatic organisms from different parts of the world. Isolates from Rohu (Labeo rohita) of Indian, California perch (Hyperprosopon ellipticum), soft-shelled turtle (Amyda cartilaginea), and Nile tilapia (Oreochromis niloticus) from Taiwan were used to test for genetic differences of A. hydrophila. In addition, we assembled a repository of A. hydrophila isolates from different aquatic organisms obtained from different parts of the world deposited in public databanks. Our findings show significant interspecies and inter-geographical variations in the 16S rRNA sequences of A. hydrophila from different aquatic organisms. Apart from 16S rRNA, we also analyzed the sequence alignments of the outer membrane protein W (OmpW) for the different aquatic organisms used in this study in order to elucidate the possibility of using this gene as a cross protective antigen against different A. hydrophila serotypes. In general, this study shows that combining 16S rRNA bacteria identification with sequence analysis of antigenic proteins for different bacteria strains of aquatic organisms could serve as an effective strategy for identifying immunogenic proteins that are across protective against different bacteria strains. These findings will be presented and discussed herein in detail. Keywords: Aeromonas hydrophila, 16S ribosomal RNA, Outer membrance, Rohu, Perch, Nile tilapia, Aquatic Organisms Conference: AquaEpi I - 2016, Oslo, Norway, 20 Sep - 22 Sep, 2016. Presentation Type: Oral Topic: Aquatic Animal Epidemiology Citation: Dubey S, Mugimba KK, Mutoloki S, Evensen O and Munang'Andu HM (2016). Molecular epidemiology of Aeromonas hydrophila based on 16 S ribosomal RNA and outer membrane protein W in different aquatic organisms. Front. Vet. Sci. Conference Abstract: AquaEpi I - 2016. doi: 10.3389/conf.FVETS.2016.02.00056 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 May 2016; Published Online: 14 Sep 2016. * Correspondence: Dr. Hetron M Munang'Andu, Norwegian University of Life Sciences, Oslo, Norway, hetron.m.munangandu@nord.no Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Saurabh Dubey Kizito K Mugimba Stephen Mutoloki Oystein Evensen Hetron M Munang'Andu Google Saurabh Dubey Kizito K Mugimba Stephen Mutoloki Oystein Evensen Hetron M Munang'Andu Google Scholar Saurabh Dubey Kizito K Mugimba Stephen Mutoloki Oystein Evensen Hetron M Munang'Andu PubMed Saurabh Dubey Kizito K Mugimba Stephen Mutoloki Oystein Evensen Hetron M Munang'Andu Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.