Abstract
BackgroundThe various virulence factors of methicillin‐resistant Staphylococcus aureus bacteremia (MRSAB) are associated with a high mortality rate worldwide. Further studies are warranted to confirm the significant relationship between the strains and virulence genes. Here, we prospectively investigated the molecular characteristics underlying the genotypes and virulence factors of MRSA isolated from patients with bacteremia.MethodsWe collected 59 MRSA isolates from adult patients with bacteremia. Antimicrobial susceptibility results were obtained with the Vitek2 automated system. Genotypes were identified with multi‐locus sequence typing (MLST) and pulse‐field gel electrophoresis (PFGE), and 21 virulence genes were detected with polymerase chain reaction (PCR).ResultsThe 59 MRSA isolates mainly comprised ST5 (n = 31, 52.5%) and ST72 (n = 22, 37.2%). Most ST5 isolates and all ST72 isolates were clustered into one and two PFGE groups, respectively. The mean number of virulence genes was higher in ST5 than in ST72. Sel was more frequently detected in ST5 than in ST72, whereas sec and sed were found only in ST5. ST5 had significantly higher resistance against many antibiotics than ST72.ConclusionMost MRSA isolates causing bacteremia were ST5 (CC5) and ST72 (CC8), and those belonging to the same STs were divided into only a few PFGE groups. ST5 was associated with higher antibiotic resistance and staphylococcal superantigen toxin genes, than ST72, which may be related to its higher virulence.
Highlights
The various virulence factors of methicillin‐resistant Staphylococcus aureus bacteremia (MRSAB) are associated with a high mortality rate worldwide
The disk diffusion test for cefoxitin was performed for all Methicillin‐resistant Staphylococcus aureus (MRSA) isolates identified by the automated system Vitek[2], and the results were con‐ firmed according to Clinical and Laboratory Standards Institute (CLSI) guideline.[18] mecA genes were detected using polymerase chain reaction (PCR).[19]
We identified ST5 and ST72 as the major strains of MRSA involved in causing bacteremia
Summary
The various virulence factors of methicillin‐resistant Staphylococcus aureus bacteremia (MRSAB) are associated with a high mortality rate worldwide. We prospectively investigated the molecular char‐ acteristics underlying the genotypes and virulence factors of MRSA isolated from patients with bacteremia. Methods: We collected 59 MRSA isolates from adult patients with bacteremia. Genotypes were identified with multi‐locus sequence typing (MLST) and pulse‐field gel electrophoresis (PFGE), and 21 virulence genes were detected with polymerase chain reaction (PCR). Results: The 59 MRSA isolates mainly comprised ST5 (n = 31, 52.5%) and ST72 (n = 22, 37.2%). ST5 had significantly higher resistance against many antibiotics than ST72. Conclusion: Most MRSA isolates causing bacteremia were ST5 (CC5) and ST72 (CC8), and those belonging to the same STs were divided into only a few PFGE groups. ST5 was associated with higher antibiotic resistance and staphylococcal superantigen toxin genes, than ST72, which may be related to its higher virulence
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