Abstract
BackgroundDengue virus (DENV) infection is the most prevalent arthropod-borne viral infection in tropical and subtropical regions worldwide. Guangzhou has the ideal environment for DENV transmission and DENV epidemics have been reported in this region for more than 30 years.MethodsInformation for DENV infection cases in Guangzhou from 2001 to 2010 were collected and analyzed. The DENV strains were cultured and isolated from patients’ sera. Viral RNA was extracted from cell culture supernatants. cDNA was synthesized by reverse transcription PCR. Phylogenetic trees of four DENV serotypes were constructed respectively.ResultsIn total, 2478 DENV infection cases were reported; 2143 of these (86.43%) occurred during 3 months of the year: August, September and October. Of these, 2398 were local cases (96.77%) and 80 were imported cases (3.23%). Among the imported cases, 69 (86.25%) were from Southeast Asian countries. From the 90 isolated strains, 66.67%, 3.33%, 14.44%, and 15.56% belonged to DENV serotypes 1, 2, 3, and 4, respectively. DENV-1 was predominant in most of the years, including during 2 outbreaks in 2002 and 2006; however, none of the strains or genotypes identified in this study were found to be predominant. Interestingly, DENV strains from different years had different origins. Moreover, the strains from each year belonged to different serotypes and/or genotypes.ConclusionsSoutheast Asia countries were found to be the possible source of DENV in Guangzhou. These findings suggest that there is increasing diversity in DENV strains in Guangzhou, which could increase the risk of DENV outbreaks in the near future.
Highlights
Dengue virus (DENV) infection is the most prevalent arthropod-borne viral infection in tropical and subtropical regions worldwide
DENV strains from different years had different origins
These findings suggest that there is increasing diversity in DENV strains in Guangzhou, which could increase the risk of DENV outbreaks in the near future
Summary
Dengue virus (DENV) infection is the most prevalent arthropod-borne viral infection in tropical and subtropical regions worldwide. Dengue virus (DENV) is a member of the genus Flavivirus, family Flaviviridae. It is an enveloped virus with an 11-kb positive sense single-stranded RNA genome. Guangzhou Center for Disease Control and Prevention, 1 Qide Road, Guangzhou, Guangdong 510440, China syndromes ranging from a severe flu-like illness called dengue fever (DF) to lethal complications like dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) [1,2]. Infection with any of the 4 serotypes can cause extremely severe manifestations. Natural infection with any of the serotypes can only provide long-term homotypic immunity, which leads to a higher risk for DHF/DSS during secondary infections with a heterogenous serotype [3,4]
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