Molecular dynamics characteristics of bladder tumor homing targeting peptide NYZL1

Abstract

Objective To study the functions and molecular dynamics characteristics of bladder tumor targeting peptide NYZL1. Methods Methyl thiazol tetrazolium (MTT) method was used to determine the function of NYZL1. Immunofluorescence techniques, tissue immunofluorescence techniques and in vivo fluorescence imaging technology were used to research the internalization of NYZL1-fluorescein Isothiocyanate (FITC) molecular probe in cells, and the distribution and kinetics of molecular probes in the tumor microcirculation and organ in vivo. Results NYZL1-FITC fluorescence separately with different concentrations is 416.00±13.20, 966.85±20.20 and 1 751.45±16.40, while fluorescence to join NYZL1 added NYZL1-FITC is 180.45±12.40, 696.30±16.30, 1 253.24±11.50. There are significant differences between the groups (P<0.05). In 25 μmol/L and 100 μmol/L concentration of NYZL1 and NYZL1-FITC, optical density is 0.613±0.055, 0.646±0.061 and 0.647±0.063, 0.664±0.070 with the relative inhibition rate of less than 30%; The probe into the cell, a green phosphor 5 min, 10-20 min the developing nuclei, the fluorescence intensity of the strongest cell on 4 h; After intravenous injection of the probe, part of the probe were excreted by the liver-gallbladder and kidney, part of it were targeted uptake to the tumor tissue via the blood circulation, after 4-6 h tumor tissue uptake reached a maximum peak, but the distribution of the probe in tissue related with the tumor blood vessels and mark with the tumor blood vessels and the cells simultaneously. Conclusion NYZL1 does not have anti-tumor effect, it can carry a fluorescent dye targeting to bladder tumor for optical molecular imaging, will be a good targeted carrier in bladder cancer diagnosis and targeted therapy. Key words: Bladder cancer; Non-muscle invasive tumors; Tumor targeting peptide; Molecular dynamics