Abstract

Medicinal plants are nature’s gifts and used in different traditional medicinal systems of India. Most of the medicinal plants are obtained from forest without proper management and knowledge of collection. For the authentication of medicinal plants from the substituted materials, a genetic diversity study is necessary. In the present investigation molecular diversity of <i>Cipadessa baccifera</i> was carried out using dominant ISSR (inter simple sequence repeats) markers in four different accessions of Western Ghats of South India. Five primers were selected from a total of ten primers based on the reproducibility of the bands. The number of polymorphic loci was 13 and the percentage of polymorphic loci was 56.52. The genetic distance among the populations ranged from 0.0910 to 0.3629 and the genetic identity ranged from 0.6087 to 0.9130. The overall observed and effective number of alleles was about 1.5652 and 1.3913. Nei’s overall genetic diversity and Shannon information index were 0.2283 and 0.3349. The dendrogram was constructed based on the UPGMA method and the clusters formed depending upon the genetic characters.

Highlights

  • Cipadessa baccifera is a shrub mainly grows in the tropical areas of Asia [1]

  • The molecular level authentication of medicinal plants is necessary with the help of molecular markers

  • In the present study Inter simple sequence repeats (ISSR) markers based on DNA fingerprinting were used to assess the genetic diversity among four different populations of Western Ghats of C. baccifera

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Summary

Introduction

Cipadessa baccifera is a shrub mainly grows in the tropical areas of Asia [1] It is one of well-known traditional medicines in India for the treatment of rheumatoid arthritis, dysentery and pruritus [2], the paste of root, leaf and bark of this plant are applied for topically to cure psoriasis [3]. In spite of the economic and medicinal values of C. baccifera no serious attention should be paid to diversity, characterization and taxonomical identification at the molecular level. This is a prerequisite to the exploitation of the vast genetic variability available for the improvement of the quality and quantity of their drug contents. In the present investigation molecular diversity of C. baccifera of four different accessions of Western Ghats of South India was carried out using dominant ISSR markers

Materials and Methods
DNA Isolation
ISSR-PCR Analysis
Agarose Gel Electrophoresis of PCR Products
Data Analysis
Results and Discussion
Conclusion
Full Text
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