Abstract
Zingiber officinale Roscoe commonly known as ginger is one of the most important spice crop of North East India. This plant has found a wide range of application- as condiments and spice, as flavouring agent, in medicine and as therapeutic uses in pharmaceuticals industries. Different varieties of Zingiber officinale are cultivated in this region but their identification is still lacking till now. Evaluation of genetic diversity is therefore essential for conservation, to avoid duplicity of germplasm and maintenance of this important species. Molecular markers Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) are tested for eighty high dry recovery genotypes. Selections of genotypes are based on more than 25% dry rhizome recovery. Among 90 primers (40 RAPD and 50 ISSR) initially tested, 26 RAPD and 28 ISSR are used in the study which showed 84.1% and 85.2% polymorphism respectively. Dendrogram generated from UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis for both RAPD and ISSR marker was similar. Similar conclusion can be drawn from the result of cluster analysis. These primers could be used for future studies as varietal developmental programme on high dry rhizome recovery lines.
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