Abstract
BackgroundBagridae is an important family of catfishes and has a high market demand. Recently, more cultivable Bagridae fishes are being exploited in China, and hybridization of some species has been carried out to achieve better growth performance, favorable sex ratios and better disease resistance. Yet, these hybrids have further increased the difficulties of taxonomy identification due to morphological indistinguishableness.ResultsIn this study, the molecular identification technologies for Tachysurus fulvidraco, Leiocassis longirostris sand their hybrids were successfully established by using mitochondrial COI and nuclear ITS sequences to identify the maternal and paternal lineage, respectively.ConclusionThese molecular diagnostic methods could also be used to manage breeding plans of hybrids, monitor and minimize the negative impacts of hybridization programs in aquaculture. Furthermore, our study could also provide a reference for establishing detection technique for hybrids in other groups of fishes.
Highlights
Bagridae is an important family of catfishes and has a high market demand
Cytochrome C Oxidase subunit I (COI) sequences were obtained by the described primer pairs in Table 1 which were designed from the COI sequences of T. fulvidraco
For the COI sequences, the fragment sizes were approximately 1500 bp and there was no obvious difference in length among T. fulvidraco, L. longirostris, FTL and FLT (Table 1)
Summary
More cultivable Bagridae fishes are being exploited in China, and hybridization of some species has been carried out to achieve better growth performance, favorable sex ratios and better disease resistance. These hybrids have further increased the difficulties of taxonomy identification due to morphological indistinguishableness. Bagridae is an important family of catfishes which belongs to Order Siluriformes and consists of more than 220 species of 21 genera (Ferraris 2007). It is commonly found in Africa and Asia and has abundant species diversity. To improve the growth performance and survival rate, some crossbreeding programs
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