Abstract
This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo. A total of 440 blood samplesfrom volunteer were collected and tested by ELISA and multiplex PCR for HBV, HCV and HIV detection. Among the 440 volunteer blood donors, 83% were female and 17% were male. Age range of 20-29 years was more represented (73%). Whereas, multiplex PCR detected more cases of HBV than ELISA (50% vs 33%, P=0.0155);ELISA more detected HCV than PCR (34% vs 3%, P<0.0001) and HIV (26% vs 7%, P<0.0001). Confirming these observations our data showed that multiplex PCR was more sensitive in the detection of HBV. The sensitivity of ELISA for the detection of HCV and HIV was elevated compared to multiplex PCR. Multiplex PCR was more specific that ELISA for the detection of HCV and HIV.Interestingly, our data showed that the gender do not influenced the sensitivity of either ELISA or multiplex PCR to detect these viruses. This study showed the limit of both ELISA and multiplex PCR in the detection of HBV, HCV and HIV.
Highlights
The persistence of the risk of viral infection transmission in blood transfusion is a serious public health problem
To improve the quality of blood products (PSL) and ensure blood safety, a systematic serological screening of Human Immuno deficiency Virus (HIV), Hepatitis B virus (HBV), and Hepatitis C Virus (HCV)were introduced in the selection of blood donors [1,2,3]
In this study we evaluated the effectiveness of multiplex real-time Polymerase Chain Reaction (PCR), that can simultaneously detect the three major viruses (HBV, HCV and HIV) diagnosed among blood donors of the National Blood Transfusion Centre of Lomé
Summary
The persistence of the risk of viral infection transmission in blood transfusion is a serious public health problem. Despite the measures taken to reduce or eliminate the transmission of these viruses by blood transfusion, the residual post-transfusion risk remains [7]. This persistence is related to the serological box during which the serological techniques are less effective. For this reason, a good transfusion policy based on rigorous screening strategies must be implemented [8]. Using the PCR in the detection of the viral genome (DGV) has the advantage of reducing the silent phase of the most sought virus in blood donors. In this study we evaluated the effectiveness of multiplex real-time PCR, that can simultaneously detect the three major viruses (HBV, HCV and HIV) diagnosed among blood donors of the National Blood Transfusion Centre of Lomé
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