Molecular Diagnosis of some Virulence Genes in Pseudomonas aeruginosa Clinical Isolates in Wasit Province

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen and is a common cause of nosocomial infection, which causes a wide range of infection, Two hundred and seventy eight samples were collected from different hospitals and private clinics in Waist province during the period from 15th September 2017 to the15th December 2017. Seventy one isolates were identification as Pseudomonas aeruginosa by culture characterized, biochemical tests, API20E. Sixty seven isolates were detected as Peudomonas aeruginosa by molecular methods of 16SrRNA gene. The polymerase chain reaction technique was used for screening, the (9) virulence genes (opr L, opr I, tox A, lasB, phzS, exoS, phzM, phzH and nan1), the result showed that 67(100%) isolates were PCR positive for oprL and oprI. The determined of toxA gene that PCR positive for 56(83.6%). Another gene lasB were 65(97%) PCR positive. phzS gene were 61(91%) PCR positive. The result of PCR showed that 50(74.6%) isolates were PCR positive for exoS gene. The phzM gene in sixty seven isolates of Pseudomonas aeruginosa give PCR positive result in 36(53.7%). Results of phzH gene in Pseudomonas aeruginosa 32(47.8%). The nan1 gene detected in 67 isolates of Pseudomonas aeruginosa and percentage of PCR positive were 48(71.6%). In conclusion, in studied the samples, still wounds are the common sites for Pseudomonas aeruginosa followed by ear and urine. 16SrRNA based PCR assay is highly accurate and reliable for identification of Pseudomonas aeruginosa.